Detection of programmed cell death using fluorescence energy transfer.
نویسندگان
چکیده
Fluorescence energy transfer (FRET) can be generated when green fluorescent protein (GFP) and blue fluorescent protein (BFP) are covalently linked together by a short peptide. Cleavage of this linkage by protease completely eliminates FRET effect. Caspase-3 (CPP32) is an important cellular protease activated during programmed cell death. An 18 amino acid peptide containing CPP32 recognition sequence, DEVD, was used to link GFP and BFP together. CPP32 activation can be monitored by FRET assay during the apoptosis process.
منابع مشابه
Impact of Duration and Severity of Persistent Pain on Programmed Cell Death
Programmed cell death is a highly regulated form of cell death, mostly distinguished by the activation of a family of cystein-aspartate proteases (caspases) that cleave various proteins resulting in morphological and biochemical changes characteristic of this form of cell death. Several recent studies have addressed the role of programmed cell death in inflammatory and chronic pain states. Casp...
متن کاملImpact of Duration and Severity of Persistent Pain on Programmed Cell Death
Programmed cell death is a highly regulated form of cell death, mostly distinguished by the activation of a family of cystein-aspartate proteases (caspases) that cleave various proteins resulting in morphological and biochemical changes characteristic of this form of cell death. Several recent studies have addressed the role of programmed cell death in inflammatory and chronic pain states. Casp...
متن کاملThe fate of Krüppel-like factor 9-positive hepatic carcinoma cells may be determined by the programmed cell death protein 5.
Liver cancer in men is the fifth most frequently diagnosed cancer worldwide. Human Krüppel-like factor (KLF9) gene, localized on human chromosome 9q13, has been implicated in mediating a diverse range of biological processes including stem cell maintenance and differentiation of T- and B-lymphocytes. In this study, we confirmed that the levels of KLF9 mRNA and protein were lower in hepatocellul...
متن کاملReal-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure.
Caspase-like proteases have been demonstrated to be involved in plant programmed cell death (PCD). Here, the time scale of caspase-3-like protease activation was investigated in single living plant cells undergoing PCD induced by ultraviolet C (UV-C) overexposure. The real-time detection of caspase-3-like protease activation was achieved by measuring the degree of fluorescence resonance energy ...
متن کاملThe Role of Caspase 9 during Programmed Cell Death in Ciliary Ganglia of Chick Embryos
During programmed cell death (PCD) apoptosis is controlled by many factors such as proteases. With no specific protease (s) known during PCD in the developing nervous system so far, we sought to determine if any specific protease (s) is involved in this process and therefore used different protease inhibitors during PCD (from embryonic day 6 to 10) in chick embryos. Among the inhibitors commerc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Nucleic acids research
دوره 26 8 شماره
صفحات -
تاریخ انتشار 1998